Ellie is a Tibetan mastiff who was inseminated in November 2012 by Sean using frozen semen. Progesterone blood samples and a vaginal cytology confirmed that she had ovulated and the egg was mature. She was inseminated at Beech House where all the reproduction staff where on hand to make it as smooth as possible. 28 days following artificial insemination Ellie was scanned which confirmed a pregnancy. This lovely bitch went on to have a litter of pups much to the delight of all involved.